The Smart Specialization Strategy effectively targets funds (European, national, regional and private) towards activities that strengthen innovation capacity and towards the priority-marked areas of those specializations striving to increase the competitiveness of the Czech Republic. In the Czech context, the output from fulfilling these preliminary conditions has been the Regional Innovation Strategies, the regional dimensions of which are provided in the 14 regional appendices, focused on the specifics of forming a regional innovation strategy for the regions in question. One aspect of these regional appendices is to detail with greater precision the national priorities in relation to science and research, and the innovation potential of that particular region. The Regional Innovation Strategy of the South Moravian Region 2014–2020 (RIS SMR) is a fundamental strategic document of the South Moravian Region and the statutory city of Brno for implementing policy to support competitiveness, founded in particular on innovation and maximizing the economic benefits of public investment into research and education. The RIS SMR was established to create the conditions for competitive, knowledge-intensive business, in particular through investment in raising standards in education and research, improving the image of the SMR and through direct support of entrepreneurial activities.